abstract：:The effects of fentanyl on ultrastructure, protein biosynthesis, and atrial natriuretic peptide (ANP) secretion were studied in neonatal rat cardiomyocytes (CM). Ventricles from 2-day-old American Wistar rats were digested with 1% collagenase in perfusion buffer. Eight hundred thousand to 1.0 million cells/ml were inc...

journal_title：Journal of molecular and cellular cardiology

authors： Mekhail NA,Doss DN,Bravo EL,Estafanous FG

更新日期：1994-04-01 00:00:00

abstract：:This study addresses the role of polyamines and their rate limiting enzyme, ornithine decarboxylase in regulation of macromolecular transport of two macromolecules, fluorescein and horseradish peroxidase, across coronary capillaries. Rat hearts were isolated and retrogradely perfused through the aorta (Langendorff met...

journal_title：Journal of molecular and cellular cardiology

authors： Trout JJ,Lu CY,Goldstone AD,Iqbal Z,Koenig H

更新日期：1994-03-01 00:00:00

abstract：:The modulating effects of Ca2+ on single K+ channel currents in canine heart sarcoplasmic reticulum were studied using a planar lipid bilayer technique. The open-state probability and the unitary open-state current both decreased gradually as the Ca2+ concentration was reduced from pCa 3 to pCa 7.5. Each single-channe...

journal_title：Journal of molecular and cellular cardiology

authors： Uehara A,Yasukochi M,Imanaga I

更新日期：1994-02-01 00:00:00

abstract：:Adaptive cardiac hypertrophy in the rat has been characterized as pathological or physiological reflecting the nature of the inciting stimulus. These two adaptations are distinguished by alterations in contractility and in the myosin ATPase composition of the affected muscle. We investigated the relative amounts of th...

journal_title：Journal of molecular and cellular cardiology

authors： Buttrick PM,Kaplan M,Leinwand LA,Scheuer J

更新日期：1994-01-01 00:00:00

abstract：:The importance of the Na+/K+/Cl- co-transport system of the rat myocardial sarcolemma was studied under hypothermic ischemia by investigating the effect of the co-transport blockers furosemide and bumetanide on the sodium influx into the myocardium. The intracellular Na+ accumulation during hypothermic ischemia was fo...

journal_title：Journal of molecular and cellular cardiology

authors： Rubin Y,Navon G

更新日期：1993-12-01 00:00:00

abstract：:To investigate the contribution of the cardiac renin-angiotensin system to ventricular dilatation after myocardial infarction, we examined the effects of 3-week treatments with an angiotensin converting enzyme inhibitor, delapril, and a selective angiotensin II type 1 (AT1) receptor antagonist, TCV-116, on haemodynami...

journal_title：Journal of molecular and cellular cardiology

authors： Yamagishi H,Kim S,Nishikimi T,Takeuchi K,Takeda T

更新日期：1993-11-01 00:00:00

abstract：:Hypothermic cardioplegic solutions are currently used to preserve cardiac function during transportation. However, it has been shown that end-diastolic compliance decreases in donor hearts during reperfusion. Excessively cold temperatures may affect membrane-bound enzymes (Ca2+ ATPase and Ca2+ uptake) which are necess...

journal_title：Journal of molecular and cellular cardiology

authors： Labow RS,Hendry PJ,Meek E,Keon WJ

更新日期：1993-10-01 00:00:00

abstract：:To examine the action of alpha-tocopherol on high energy phosphate compounds, a 31P-NMR technique was applied to perfused Langendorff rat hearts. Rats were treated with tocopherol acetate (25 mg/kg body wt i.p.) for 7 consecutive days. On the 7th day, the rat hearts were isolated for the Langendorff experiment. After ...

journal_title：Journal of molecular and cellular cardiology

authors： Kotegawa M,Sugiyama M,Shoji T,Haramaki N,Ogura R

更新日期：1993-09-01 00:00:00

abstract：:Reperfusion or reoxygenation of ischaemic or hypoxic cardiac tissue results in increases in total cell calcium and cell lysis both of which are dependent on mitochondrial function. Although changes which occur during this period of exposure of the tissue to hypoxia predispose the heart to take up calcium on reoxygenat...

journal_title：Journal of molecular and cellular cardiology

authors： Allen SP,Darley-Usmar VM,McCormack JG,Stone D

更新日期：1993-08-01 00:00:00

abstract：:This study examined the potential role of ET-1 and the contribution of protein kinase C (PKC) in the desensitization of the ET-1 transmembrane signaling pathway in the left circumflex coronary artery (CCA) of a dog model of congestive heart failure (CHF). In the CCA of the rapid ventricular pacing-overdrive dog model ...

journal_title：Journal of molecular and cellular cardiology

authors： Calderone A,Rouleau JL,de Champlain J,Bélichard P,Stewart DJ

更新日期：1993-08-01 00:00:00

abstract：:Under normal physiological conditions atrial natriuretic factor (ANF) in rat is stored in secretory granules in fetal and adult atrial cardiomyocytes (Cantin et al., 1984; de Bold, 1985), whereas in fetal and neonatal ventricular cardiomyocytes secretory granules are practically absent and ANF is directly secreted via...

journal_title：Journal of molecular and cellular cardiology

authors： Eppenberger-Eberhardt M,Messerli M,Eppenberger HM,Reinecke M

更新日期：1993-07-01 00:00:00

abstract：:Endothelial cells produce endothelin, a powerful vasoconstrictor. We report the release of additional vasoconstrictor material in conditional filtrate from freshly harvested cells, which we identified as leukotrienes by radioimmunoassay (RIA) and by high pressure liquid chromatography (HPLC). The material was collecte...

journal_title：Journal of molecular and cellular cardiology

authors： Kähler J,Conforto A,Dudek R,Termin A,Bing RJ

更新日期：1993-06-01 00:00:00

abstract：:Studies to test whether superoxide dismutase (SOD), with or without catalase, limits myocardial infarct size have produced conflicting results. Positive results following short periods of reperfusion vs negative results following longer periods of reperfusion could be explained if either: (1) myocytes, initially salva...

journal_title：Journal of molecular and cellular cardiology

authors： Tanaka M,Richard VJ,Murry CE,Jennings RB,Reimer KA

更新日期：1993-04-01 00:00:00

abstract：:Hearts from untreated or enalapril-treated piglets were used to measure rates of ribosome formation and total protein synthesis during perfusion as modified Langendorff preparations. Pretreatment of newborn piglets with enalapril maleate (5 mg, once daily for 3 days) resulted in a decreased rate of growth of the left ...

journal_title：Journal of molecular and cellular cardiology

authors： Beinlich CJ,Baker KM,Morgan HE

更新日期：1993-04-01 00:00:00

abstract：:The present study was undertaken to assess whether [32P]uptake and autoradiography could be used as an indicator of regional myocardial oxygen consumption. Rat hearts were perfused in the Langendorff mode with medium containing [32P]orthophosphate (32Pi) and the incorporation of label into ATP, perchloric acid-insolub...

journal_title：Journal of molecular and cellular cardiology

authors： Richards SM,Rattigan S,Colquhoun EQ,Clark MG

更新日期：1993-03-01 00:00:00

abstract：:The purpose of this study was to investigate whether vitamin D3 deficiency affects the cardiac function of chick hearts directly or whether the influence is secondary through the hormone's effect on serum calcium levels. To this end, three experimental groups were studied: (a) the control group of vitamin D3 supplemen...

journal_title：Journal of molecular and cellular cardiology

authors： Hochhauser E,Kushnir T,Navon G,Rehavi M,Barak J,Edelstein S,Vidne B

更新日期：1993-01-01 00:00:00

abstract：:Histamine is synthetized in the heart, and released by ischemia-reperfusion injury in several species. Histamine has arrhythmogenic, chronotropic, inotropic and vasoactive effects. Cardiac histamine release during ischemia-reperfusion may be mediated by toxic oxygen metabolites. We studied the effect of ischemia-reper...

journal_title：Journal of molecular and cellular cardiology

authors： Valen G,Kaszaki J,Szabó I,Nagy S,Vaage J

更新日期：1993-01-01 00:00:00

abstract：:The effect of taurine on phenylephrine alpha-adrenergic action was studied in freshly-isolated guinea-pig ventricular myocytes. Intracellular calcium concentration was measured at nearly physiological extracellular CaCl2 in both cell suspension using quin-2 (mean intracellular calcium concentration 154.0 +/- 8.0 nM, n...

journal_title：Journal of molecular and cellular cardiology

authors： Failli P,Fazzini A,Franconi F,Stendardi I,Giotti A

更新日期：1992-11-01 00:00:00

abstract：:With a research hypothesis that the behavior of blood perfused hearts was different from that of crystalloid perfused hearts, we tested the null hypothesis that the functional and metabolic status of blood-perfused (paracorporeal oxygenation) and Krebs-Henseleit (bubble oxygenation) perfused Langendorff isolated rat h...

journal_title：Journal of molecular and cellular cardiology

authors： Walters HL 3rd,Digerness SB,Naftel DC,Waggoner JR 3rd,Blackstone EH,Kirklin JW

更新日期：1992-10-01 00:00:00

abstract：:In mammalian myocardium, muscle contraction is regulated by the rapid release of Ca2+ ions through ryanodine-sensitive Ca2+ release channels present in the intracellular membrane compartment, sarcoplasmic reticulum (SR). In this study, the effects of regional ischemia on intrinsic SR Ca2+ release channel function were...

journal_title：Journal of molecular and cellular cardiology

authors： Darling EM,Lai FA,Meissner G

更新日期：1992-10-01 00:00:00

abstract：:The adult newt cardiac ventricular myocyte has been successfully placed in cell culture and has been shown to undergo in vitro DNA synthesis. Although several growth factors have been reported to increase DNA synthesis in cardiac myocytes in vitro, PDGF has not been reported to do so, but has been shown to be active i...

journal_title：Journal of molecular and cellular cardiology

authors： Soonpaa MH,Oberpriller JO,Oberpriller JC

更新日期：1992-09-01 00:00:00

abstract：:The agent 2,3-butanedione monoxime (BDM) has been reported to reduce the sensitivity of myofilament force development to calcium ions, without affecting the calcium transient in myocardium. One would predict, therefore, that BDM should reduce the contractile state of the heart without reducing the amount of oxygen tha...

journal_title：Journal of molecular and cellular cardiology

authors： de Tombe PP,Burkhoff D,Hunter WC

更新日期：1992-08-01 00:00:00

abstract：:The effects of variations in serum concentrations of potassium on the occurrence and severity of ischemia- and reperfusion-induced arrhythmias have been studied in conscious rats. Serum potassium concentrations were modified by maintaining rats on diets which varied in potassium concentration, by treatment with hydroc...

journal_title：Journal of molecular and cellular cardiology

authors： Saint KM,Abraham S,MacLeod BA,McGough J,Yoshida N,Walker MJ

更新日期：1992-07-01 00:00:00

abstract：:Myocardial ischemia is associated with accumulation of lyso-phospholipids, including lyso-platelet activating factor, the degradation product and precursor of platelet activating factor. These compounds produce cellular and microvascular damage and, in the myocardium, depression of contractility and arrhythmia. The po...

journal_title：Journal of molecular and cellular cardiology

authors： Leong LL,Sturm MJ,Papadimitriou JM,Stephens CJ,Taylor RR

更新日期：1992-06-01 00:00:00

abstract：:Studies using chemically-induced models of diabetes have shown the diabetic myocardium to exhibit abnormalities in cellular ion transport, which may affect susceptibility to reperfusion-induced arrhythmias. We studied the incidence of reperfusion-induced ventricular tachycardia (VT) and fibrillation (VF) in isolated h...

journal_title：Journal of molecular and cellular cardiology

authors： Kusama Y,Hearse DJ,Avkiran M

更新日期：1992-04-01 00:00:00

abstract：:Release of adenosine and AMP into epicardial fluid and coronary venous effluent of isovolumic guinea-pig hearts was examined during normoxic (95% O2) and hypoxic (30% O2) perfusion with and without the ecto-5'-nucleotidase inhibitor alpha,beta-methylene adenosine diphosphate (AOPCP)*. Normoxic epicardial and venous ad...

journal_title：Journal of molecular and cellular cardiology

authors： Headrick JP,Matherne GP,Berne RM

更新日期：1992-03-01 00:00:00

abstract：:The correlations between myocardial redox and energy states and atrial natriuretic peptide (ANP) secretion were studied in the perfused rat heart by exposing the hearts to global and low-flow ischemia for varying periods. Atrial and ventricular energy states and immunoreactive ANP in the effluent perfusate were measur...

journal_title：Journal of molecular and cellular cardiology

authors： Uusimaa PA,Peuhkurinen KJ,Vuolteenaho O,Ruskoaho H,Hassinen IE

更新日期：1992-02-01 00:00:00

abstract：:The effects of adenosine on myocardial substrate utilization were assessed in isolated Langendorff-perfused rat hearts. In unpaced hearts perfused at constant flow with insulin, adenosine inhibited oxidation of both glucose and palmitate with similar dose-dependency, without significant effects on effluent lactate+pyr...

journal_title：Journal of molecular and cellular cardiology

authors： Buxton DB,Kjaer-Pedersen K,Nguyen A

更新日期：1992-02-01 00:00:00

abstract：:The neuropeptide galanin (GAL) has been detected in the peripheral and central nervous systems. However, little is known about its distribution and localization in heart, and the possible coexistence of GAL with other neuropeptides in the heart is not established. The present immunocytochemical study describes the dis...

journal_title：Journal of molecular and cellular cardiology

authors： Zhu W,Dey RD

更新日期：1992-01-01 00:00:00

abstract：:The question was investigated whether mild acidosis can provide energetic protection on the hypoxic myocardial cell by mechanisms other than negative inotropy. The experimental model used was quiescent ventricular cardiomyocytes isolated from the adult rat. The extracellular pH (pHe) in modified hypoxic Tyrode's solut...

journal_title：Journal of molecular and cellular cardiology

authors： Koop A,Piper HM

更新日期：1992-01-01 00:00:00

abstract：:Myocardium which has been preconditioned by one or several brief episodes of ischemia has much slower energy utilization during a subsequent sustained episode of ischemia. Since preconditioned tissue also is 'stunned', the reduced energy utilization of preconditioned tissue may be due to reduced contractile effort. Th...

journal_title：Journal of molecular and cellular cardiology

authors： Jennings RB,Murry CE,Reimer KA

更新日期：1991-12-01 00:00:00

abstract：:The binding properties of [3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [3H]U69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association an...

journal_title：Journal of molecular and cellular cardiology

authors： Tai KK,Jin WQ,Chan TK,Wong TM

更新日期：1991-11-01 00:00:00

abstract：:The failure of adenosine receptor antagonists to consistently attenuate metabolic coronary vasodilation suggests that adenosine is not a primary regulator of functional hyperemia. An alternative hypothesis, however, is that metabolic stimulation of the heart in the presence of an adenosine receptor antagonist results ...

journal_title：Journal of molecular and cellular cardiology

authors： Heller LJ,Dole WP,Mohrman DE

更新日期：1991-08-01 00:00:00

abstract：:Previous studies have demonstrated that in different cardiac preparations action potential duration (APD) increases with age. As in various species, thyroid hormone levels increase developmentally, and since hyperthyroidism shortens APD while hypothyroidism prolongs it, we hypothesized that developmental changes in AP...

journal_title：Journal of molecular and cellular cardiology

authors： Felzen B,Rubinstein I,Lotan R,Binah O

更新日期：1991-07-01 00:00:00

abstract：:Recently discovered endothelin is a powerful vasoconstrictor of vascular smooth muscle in vitro, which also profoundly affects the mechanical function of the heart. An increased level of plasma endothelin in patients with acute myocardial infarction suggests the enhanced release of endothelin from vascular endothelium...

journal_title：Journal of molecular and cellular cardiology

authors： Prasad MR,Jones RM,Kreutzer DL

更新日期：1991-06-01 00:00:00

abstract：:Catecholamines increase the amplitudes of oscillatory afterpotentials (OAP) and peak magnitude of the transient inward current (Iti) responsible for OAP. The objectives of this study were to determine whether beta-adrenoceptor stimulation can induce Iti, and to determine the mechanism by which beta-adrenoceptor stimul...

journal_title：Journal of molecular and cellular cardiology

authors： Han XQ,Ferrier GR

更新日期：1991-05-01 00:00:00

abstract：:The effects of N-n-propylajmaline (prajmalium) on the Na and Ca currents of single frog atrial and ventricular cells were studied by means of the whole-cell patch-clamp technique. Prajmalium (10(-9) to 10(-6) M) depressed the Na current (INa) in a dose- and use-dependent manner. In the same range of concentrations, pr...

journal_title：Journal of molecular and cellular cardiology

authors： Alvarez JL,Vassort G

更新日期：1991-05-01 00:00:00

abstract：:Human hearts with idiopathic dilated cardiomyopathy have diminished adenylate cyclase activity and increased amounts of the alpha-subunit of the inhibitory guanine nucleotide-binding regulatory protein (alpha Gi) as measured by pertussis toxin catalyzed ADP-ribosylation. We utilized specific antisera against synthetic...

journal_title：Journal of molecular and cellular cardiology

authors： Feldman AM,Jackson DG,Bristow MR,Cates AE,Van Dop C

更新日期：1991-04-01 00:00:00

abstract：:Elevation of cytosolic sodium is thought to be correlated with an increase in force of contraction due to an activation of sodium-calcium exchange. We investigated the inotropic response mediated by the new sodium-channel activator BDF 9148 (0.01-100 mumol/l) on failing human myocardium. Force of contraction was studi...

journal_title：Journal of molecular and cellular cardiology

authors： Schwinger RH,Böhm M,Mittmann C,La Rosée K,Erdmann E

更新日期：1991-04-01 00:00:00

abstract：:The instability of the oxalate-supported Ca2+ uptake activity of rat cardiac sarcoplasmic reticulum (CSR) in ventricular homogenates most likely accounts for the low specific activity of the rate of oxalate-supported Ca2+ uptake in previously reported fractions of isolated rat CSR. We have found that CSR vesicles with...

journal_title：Journal of molecular and cellular cardiology

authors： Feher JJ,Davis MD

更新日期：1991-03-01 00:00:00